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Am J Ophthalmol. 1999 Aug;128(2):237-8.

Technique for harvesting keratolimbal allografts from corneoscleral buttons.

Author information

1
Department of Ophthalmology, University of California, Davis, Sacramento, USA. mjmannis@ucdavis.edu

Abstract

PURPOSE:

To report a method for obtaining thin keratolimbal lenticules from cadaveric corneoscleral buttons for corneal epithelial stem cell allografts.

METHOD:

A standard silicone orbital sizing sphere and three 25-gauge needles were employed to fix the corneoscleral button to facilitate excision of keratolimbal lenticules.

RESULT:

Limbal stem cells in the form of keratolimbal lenticules were obtained without difficulty.

CONCLUSION:

With this technique, thin keratolimbal lenticules of good quality can be obtained efficiently, inexpensively, and without specialized instruments.

PMID:
10458185
[Indexed for MEDLINE]

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