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J Allergy Clin Immunol. 1999 Aug;104(2 Pt 1):279-84.

Syk deficiency in nonreleaser basophils.

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1
Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, USA.

Abstract

BACKGROUND:

Peripheral blood basophils from 10% to 20% of donors fail to degranulate in response to cross-linking the high-affinity IgE receptor FcepsilonRI. The molecular mechanisms underlying the nonreleaser phenotype have not been established.

OBJECTIVES:

Our aim was to compare the expression of FcepsilonRI-associated protein tyrosine kinases between nonreleaser and releaser basophils.

METHODS:

With use of Western blotting we investigated Syk and Lyn protein levels in highly purified basophils from 3 anti-IgE nonreleasers and 2 releasers.

RESULTS:

We identified 3 healthy nonatopic donors whose nonreleaser basophils express FcepsilonRI normally but fail to express protein for the tyrosine kinase Syk, which is implicated in the initiation of FcepsilonRI-mediated secretion. Protein levels for the tyrosine kinase Lyn are somewhat reduced but not absent in nonreleaser basophils. Levels of Lyn and Syk protein are similar in B cells, eosinophils, and neutrophils from releaser and nonreleaser donors. During these studies one nonreleaser "converted" into a releaser with concomitant basophil Syk expression.

CONCLUSION:

The absence of detectable Syk could explain the nonreleaser phenotype of basophils from some donors.

PMID:
10452745
[Indexed for MEDLINE]
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