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Zhongguo Yao Li Xue Bao. 1999 Mar;20(3):279-82.

Velvet antler polypeptides promoted proliferation of chondrocytes and osteoblast precursors and fracture healing.

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1
Research Centre of New Drug, Changchun College of Traditional Chinese Medicine, China.

Abstract

AIM:

To study the effects of velvet antler (VA) total polypeptides (VATP) and VA polypeptides, VAP-A, VAP-B, and VAP-C on proliferation of chondrocytes and osteoblast precusors.

METHODS:

Chondrocytes (rabbit and human fetus) and osteoblast precusors (chick embryo) were incubated in the culture medium containing VATP or VAP-A, VAP-B, and VAP-C. [3H]TdR incorporation into DNA was measured. Fracture healing-promoting action of VATP was determined in rats.

RESULTS:

VATP 50-200 mg.L-1 and VAP-B 12.5, 25, and 50 mg.L-1 showed most marked proliferation-promoting activity for rabbit costed chondrocytes and increased incorporation of [3H]TdR from (73 +/- 9) Bq (control group) to (272 +/- 55), (327 +/- 38), and (415 +/- 32) Bq, respectively (P < 0.01). The activity of VAP-A was weaker than that of VAP-B, and VAP-C had no activity. VATP 10 and 20 mg.kg-1 by local injection into the cross-section fracture area accelerated healing of radial fracture. The healing rate of VATP-treated group was higher (75%) than that of control group (25%) (P < 0.05).

CONCLUSION:

VATP accelerated fracture healing by stimulating proliferation of chondrocytes and osteoblast precursors.

PMID:
10452108
[Indexed for MEDLINE]
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