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Mol Med. 1999 Apr;5(4):224-31.

Efficient generation of recombinant adenoviral vectors by Cre-lox recombination in vitro.

Author information

1
Howard Hughes Medical Institute, University of Michigan Medical Center, Department of Internal Medicine and Biological Chemistry, Ann Arbor 48109-0650, USA.

Abstract

BACKGROUND:

Although recombinant adenovirus vectors are attractive for use in gene expression studies and therapeutic applications, the construction of these vectors remains relatively time-consuming. We report here a strategy that simplifies the production of adenoviruses using the Cre-loxP system.

MATERIALS AND METHODS:

Full-length recombinant adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA.

RESULTS:

After transfection of Cre-treated DNA into 293 cells, replication-defective viral vectors were rapidly obtained without detectable wild-type virus.

CONCLUSION:

This system facilitates the development of recombinant adenoviral vectors for basic and clinical research.

PMID:
10448644
PMCID:
PMC2230325
[Indexed for MEDLINE]
Free PMC Article

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