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J Enzyme Inhib. 1999;14(3):239-50.

Dual inhibitory effects of dimethyl sulfoxide on poly(ADP-ribose) synthetase.

Author information

1
Laboratory of Molecular Clinical Chemistry, Kyoto University, Japan.

Abstract

Dimethyl sulfoxide (DMSO), a solvent popularly used for dissolving water-insoluble compounds, is a weak inhibitor of poly(ADP-ribose) synthetase, that is a nuclear enzyme producing (ADP-ribose)n from NAD+. The inhibitory mode and potency depend on the concentration of substrate, NAD+, as well as the temperature of the reaction; at micromolar concentrations of NAD+, the inhibition by DMSO is biphasic at 37 degrees C, but is monophasic and apparently competitive with NAD+ at 25 degrees C. DMSO, on the other hand, diminishes dose-dependently and markedly the inhibitory potency of benzamide and other inhibitors. Other organic solvents, ethanol and methanol, also show a biphasic effect on the synthetase activity at different concentrations.

PMID:
10445046
DOI:
10.3109/14756369909030319
[Indexed for MEDLINE]

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