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Alcohol Clin Exp Res. 1999 Jul;23(7):1262-71.

Identification and confirmation of quantitative trait loci regulating alcohol consumption in congenic strains of mice.

Author information

1
Alcohol Research Center, Institute for Behavioral Genetics, University of Colorado, Boulder 80309-0447, USA. Valerie.Whatley@colorado.edu

Abstract

BACKGROUND:

C57BL/6 inbred mice prefer alcohol whereas DBA/2 mice avoid it. We describe the construction of congenic strains of mice in which DBA/2 alleles for alcohol avoidance were placed on a C57BL/6 background using phenotypic selection.

METHODS:

Mice were primed to drink 10% v/v ethanol in water for 2 days before a two-bottle choice paradigm. N2 males who demonstrated an alcohol-avoidance phenotype were backcrossed to B6 females to construct 15 independent lines.

RESULTS:

Eight of these lines were lost due to failure to breed or absence of males with an alcohol-avoidance phenotype. The remaining sublines were split to form a total of 21 sublines. In the N7 and N9 generations, a genome scan located provisional quantitative trait loci (QTLs) on chromosomes 1, 2, 3, 6, and 9. Progeny testing confirmed QTLs on chromosomes 1 and 2.

CONCLUSIONS:

The QTL on chromosome 2 overlaps the 95% confidence interval of Alcp1 whereas that on chromosome 1 is new and has been called Alcp5. Marker-assisted selection was used in the N9 and subsequent generations to maintain the congenic lines and produce congenic strains.

PMID:
10443995
[Indexed for MEDLINE]
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