Little literature is available on professional musical theater female singers, a population that regularly uses a wide variety of vocal qualities. This study tested the hypothesis that different vocal qualities cause observable specific configurations of muscular movements and structural changes of the larynx, hypopharynx, oral pharynx, and oral cavity for individual singers. Fiberoptic rigid and flexible endoscopic observation were used to determine visual analysis of such configurations. This study documents observable physiologic changes that were made by professional musical theater female singers in specific vocal qualities.