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Antivir Chem Chemother. 1999 May;10(3):135-9.

Mouse model of respiratory syncytial virus infection to evaluate antiviral activity in vivo.

Author information

1
Rational Drug Design Laboratories, Fukushima, Japan. sudou.kenji@yamanouchi.co.jp

Abstract

In this study, we have developed a practical mouse model for evaluating in vivo the antiviral activity of compounds against respiratory syncytial virus (RSV) infection. BALB/c mice are not particularly susceptible to RSV infection; however, infection rates were improved by pretreatment with the immunosuppressive agent cyclophosphamide (CYP). When mice were inoculated intranasally with RSV A2 strain, the pulmonary RSV titres of CYP-pretreated 10-week-old mice were higher than those of untreated 10-week-old and 28-week-old mice, peaking on days 4 and 5 post-infection. Sections of lung from RSV-infected mice pretreated with CYP, taken on day 4 post-inoculation, showed widespread evidence of interstitial pneumonia and other significant pathological changes. We also confirmed that ribavirin, a representative antiviral agent, significantly reduced the pulmonary RSV titres of mice pretreated with CYP when administered intraperitoneally.

PMID:
10431613
DOI:
10.1177/095632029901000305
[Indexed for MEDLINE]

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