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J Magn Reson. 1999 Aug;139(2):430-3.

Triple-resonance experiments for correlation of H5 and exchangeable pyrimidine base hydrogens in (13)C,(15)N-labeled RNA.

Author information

1
Abteilung für Molekulare Biophysik/NMR-Spektroskopie, Institut für Molekulare Biotechnologie e.V., Postfach 100813, Jena, D-07708, Germany.

Abstract

Triple-resonance two-dimensional H5(C5C4N)H experiments are described that provide through-bond H5 to imino/amino connectivities in uridines and cytidines in (13)C, (15)N-labeled RNAs. The experiments employ selective INEPT steps for transferring magnetization from the H5 hydrogens through the intervening C5, C4, and N3/N4 nuclei to the imino/amino hydrogens. The improved sensitivity of these experiments for assignments in a large 43-nucleotide RNA is demonstrated.

PMID:
10423381
DOI:
10.1006/jmre.1999.1797
[Indexed for MEDLINE]

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