Format

Send to

Choose Destination
Mol Microbiol. 1999 Aug;33(3):583-9.

PDZ domains determine the native oligomeric structure of the DegP (HtrA) protease.

Author information

1
Unité de Programmation Moléculaire et de Toxicologie Génétique/CNRS-URA1444, Département des Biotechnologies, Institut Pasteur, 25, rue du Docteur Roux, 75015 Paris, France.

Abstract

DegP (HtrA) is a periplasmic heat shock serine protease of Escherichia coli that degrades misfolded proteins at high temperatures. Biochemical and biophysical experiments have indicated that the purified DegP exists as a hexamer. To examine whether the PDZ domains of DegP were required for oligomerization, we constructed a DegP variant lacking both PDZ domains. This truncated variant, DegPDelta, exhibited no proteolytic activity but exerted a dominant-negative effect on growth at high temperatures by interfering with the functional assembly of oligomeric DegP. Thus, the PDZ domains contain information necessary for proper assembly of the functional hexameric structure of DegP.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center