Abstract
p53 exerts important physiological functions in cell-cycle control, gene regulation, cell differentiation, apoptosis and tumor suppression by interacting with many cellular proteins. Using the yeast two-hybrid system, we screened a HeLa cDNA library and identified a novel gene encoding a p53-binding protein (p53BP3). The full-length cDNA of p53BP3 was isolated from a HeLalambdagt10 cDNA library. This predicted protein was composed of 815 amino acids. Sequence analysis indicated that p53BP3 contained two bipartite nuclear localization signals and was confirmed to be a nuclear protein. FISH mapping results showed that this novel gene was located at human chromosome 12, region p11.2-p12.1. Northern blot analysis suggested that p53BP3 was broadly expressed in human tissues. A further study showed that p53BP3 had a homologue in mouse.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism*
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Chromosomes, Human, Pair 12 / genetics
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Cloning, Molecular
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DNA-Binding Proteins*
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Genes*
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HeLa Cells
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Humans
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Leucine Zippers
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Mice
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Molecular Sequence Data
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Neoplasm Proteins*
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Nuclear Localization Signals
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Physical Chromosome Mapping
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Protein Binding
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Sequence Deletion
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Transcription Factors*
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Transfection
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Tumor Cells, Cultured
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism*
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Ubiquitin-Protein Ligases
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Yeasts / genetics
Substances
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Carrier Proteins
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DNA-Binding Proteins
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Neoplasm Proteins
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Nuclear Localization Signals
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Nuclear Proteins
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RNA, Messenger
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Recombinant Fusion Proteins
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Transcription Factors
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Tumor Suppressor Protein p53
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TOPORS protein, human
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Ubiquitin-Protein Ligases