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Viral Immunol. 1999;12(2):97-105.

Recombinant vaccinia viruses protect against Clostridium perfringens alpha-toxin.

Author information

1
Defence Evaluation and Research Agency, Salisbury, Wiltshire, United Kingdom.

Abstract

Recombinant vaccinia viruses that expressed the nontoxic C-domain of Clostridium perfringens alpha-toxin were constructed. The J2R (thymidine kinase [TK] gene) and B13R (serpin 2 [SPI-2] gene) loci were used as insertion sites for the clostridial DNA, and expression of the foreign protein was measured in each case. A double recombinant that encoded the alpha-toxin truncate at the B13R locus and the protective antigen of Bacillus anthracis at the J2R locus was also constructed. Although differences in expression of the alpha-toxin C-domain were recorded, all of the vaccinia recombinants protected mice against a lethal challenge with alpha-toxin demonstrating that a recombinant vaccinia virus can be used to provide protection against a toxin challenge that is known to be solely antibody mediated.

PMID:
10413356
DOI:
10.1089/vim.1999.12.97
[Indexed for MEDLINE]

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