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Clin Exp Metastasis. 1999 Mar;17(2):177-81.

Experimental metastasis is suppressed in MMP-9-deficient mice.

Author information

1
Shionogi Institute for Medical Science, Shionogi & Co., Ltd, Osaka, Japan. takeshi.itoh@shionogi.co.jp

Abstract

Matrix metalloproteinases (MMPs) are thought to play a key role in tumor invasion and metastasis. The role of MMP-9 (gelatinase B) in tumor metastasis was examined in MMP-9-deficient mice produced by gene targeting using embryonic stem cells. MMP-9-deficient mice develop normally and are fertile. In these mice, the number of metastatic colonies of B16-BL6 melanoma cells or Lewis lung carcinoma cells that were implanted intravenously fell by 45% for B16-BL6 melanoma and 59% for Lewis lung carcinoma (p = 0.03 and p = 0.0043, respectively). Gelatin zymography showed that both tumor cell lines did not secrete MMP-9 by themselves but the host cells surrounding the tumor cells secrete MMP-9 in vivo. These results indicated that host-derived MMP-9 plays an important role in the process of tumor metastasis.

PMID:
10411111
[Indexed for MEDLINE]

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