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Chemosphere. 1999 Aug;39(3):467-76.

Development of an optimal bacterial medium based on the growth inhibition assay with Vibrio fischeri.

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  • 1Staatliches Umweltamt Köln-Aussenstelle Bonn-, Germany.


Chronic toxicity level of chemicals to bacteria can depend on composition and concentration of medium ingredients. This was demonstrated by means of a growth rate inhibition test with the marine bacterium V. fischeri. In a minimal medium (following the validity criterion of achieving a least cell multiplication rate) containing only yeast extract as organic nutrient component, V. fischeri was to Cu2+ 11.9 times, to Hg2+ 3 times and to Zn2+ 2.8 times more sensitive than in the complete defined medium. Obviously yeast extract did not interfere greatly in the complexation mechanisms. The detection limits of the toxicity of Cd2+, 3,5-dichorophenol and nitrobenzene were not influenced by the substrate.

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