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Infect Control Hosp Epidemiol. 1999 Jun;20(6):432-4.

Monitoring rotavirus environmental contamination in a pediatric unit using polymerase chain reaction.

Author information

1
Laboratoire de Virologie Médicale Moléculaire, Faculté de Médecine Grenoble, France.

Abstract

Rotavirus environmental contamination in a pediatric unit was investigated. Surfaces were swabbed, then viruses eluted, ultracentrifuged, and detected by polymerase chain reaction (PCR) amplification. Of 55 samples, 25 (46%) tested positive. Rotavirus RNA was more prevalent on surfaces in direct contact with children (thermometers and play mats) than on other environmental surfaces (washbasins, door handles, etc). PCR has proved useful for monitoring rotavirus environmental contamination.

PMID:
10395149
DOI:
10.1086/501648
[Indexed for MEDLINE]

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