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Ann Clin Biochem. 1999 Mar;36 ( Pt 2):196-201.

Three-colour, one-tube flow cytometric method for enumeration of CD4+ and CD8+ lymphocytes.

Author information

1
Department of Clinical Laboratories, Catharina Hospital, Eindhoven, The Netherlands.

Abstract

We investigated the usefulness of a one-tube, three-colour flow cytometric method for enumerating CD4+ and CD8+ lymphocytes. This method does not use any control antibodies and we compared it to the standard methods using either control and CD14/CD45 antibodies or control antibodies only on 38 blood samples from healthy and human immunodeficiency virus-infected patients. The one-tube method showed good agreement with the other, more complicated methods and is therefore suitable for reliable enumeration of CD4+ and CD8+ T-lymphocytes.

PMID:
10370736
DOI:
10.1177/000456329903600210
[Indexed for MEDLINE]

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