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Mol Cell Probes. 1999 Jun;13(3):175-8.

Enhancement of Mycoplasma bovis detection in milk samples by antigen capture prior to PCR.

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1
Division 4, Federal Institute for Health Protection of Consumers and Veterinary Medicine, Naumburger Str. 96a, D-07743 Jena, Germany.

Abstract

A rapid and sensitive procedure to detect Mycoplasma bovis from milk samples was developed. Mycoplasma cells were collected by selective binding to a monoclonal antibody, thus separating them from milk proteins and facilitating DNA extraction. Polymerase chain reaction (PCR) using species-specific primers allowed detection of 20 cfu ml-1 in agarose gels and 2 cfu ml-1 on Southern blots. The method is especially suitable for specimens from subclinical cases and intermittent shedders.

PMID:
10369741
DOI:
10.1006/mcpr.1999.0234
[Indexed for MEDLINE]
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