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J Clin Microbiol. 1999 Jul;37(7):2350-1.

Inactivation of Mycobacterium tuberculosis for DNA typing analysis.

Author information

1
Department of Microbiology, Centre Hospitalier Universitaire, Nantes, France.

Abstract

DNA fingerprinting analysis of Mycobacterium tuberculosis is used for epidemiological studies and the control of laboratory cross-contamination. Because standardized procedures are not entirely safe for mycobacteriology laboratory staff, the paper proposes a new technique for the processing of specimens. The technique ensures the inactivation of M. tuberculosis before DNA extraction without the loss of DNA integrity. The control of inactivated cultures should be rigorous and should involve the use of two different culture media incubated for at least 4 months.

PMID:
10364613
PMCID:
PMC85159
[Indexed for MEDLINE]
Free PMC Article

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