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Curr Opin Chem Biol. 1999 Jun;3(3):284-90.

Protein evolution by molecular breeding.

Author information

1
Maxygen Incorporated, 515 Galveston Drive, Redwood City, CA 94063, USA. jeremy_minshull@maxygen.com

Abstract

Natural evolution has guided the development of 'molecular breeding' processes used in the laboratory for the rapid modification of subgenomic sequences including single genes. The most significant recent development has been the in vitro permutation of natural diversity. Homologous recombination of multiple related sequences produced high-quality libraries of chimeric sequences encoding proteins with functions that differ dramatically from any of the parents. Increasingly powerful screening methods are also being developed, allowing these libraries to be screened for novel biocatalysts.

PMID:
10359711
DOI:
10.1016/s1367-5931(99)80044-1
[Indexed for MEDLINE]

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