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Br J Haematol. 1999 Jun;105(3):673-5.

An improved micro-method for obtaining chromosome preparations from individual haemopoietic colonies.

Author information

1
Radiation and Genome Stability Unit, Medical Research Council, Harwell, Oxfordshire.

Abstract

A robust method for obtaining chromosome preparations from individual haemopoietic colonies in semi-solid media is described. The accumulation of metaphases and the hypotonic treatment of cells were carried out in the culture dish and individual colonies were transferred onto poly-L-lysine-treated slides and fixed stepwise prior to staining. The technique produced high yields of well-spread metaphases facilitating clonal cytogenetic analysis.

PMID:
10354130
[Indexed for MEDLINE]

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