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Biotechniques. 1999 Apr;26(4):680-2.

Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis.

Author information

1
Schering-Plough Research Institute, Kenilworth, NJ 07033, USA.

Abstract

We developed a two-stage procedure, based on the QuikChange Site-Directed Mutagenesis Protocol, that significantly expands its application to a variety of gene modification experiments. A pre-PCR, single-primer extension stage before the standard protocol allows the efficient introduction of not only point mutation but also multiple mutations and deletions and insertions to a sequence of interest.

PMID:
10343905
[Indexed for MEDLINE]

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