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Cell. 1999 May 14;97(4):481-90.

The highly ordered double-stranded RNA genome of bluetongue virus revealed by crystallography.

Author information

1
The Laboratory of Molecular Biophysics, University of Oxford, United Kingdom.

Abstract

The concentration of double-stranded RNA within the bluetongue virus core renders the genome segments liquid crystalline. Powder diffraction rings confirm this local ordering with a 30 A separation between strands. Determination of the structure of the bluetongue virus core serotype 10 and comparison with that of serotype 1 reveals most of the genomic double-stranded RNA, packaged as well-ordered layers surrounding putative transcription complexes at the apices of the particle. The outer layer of RNA is sufficiently well ordered by interaction with the capsid that a model can be built and extended to the less-ordered inner layers, providing a structural framework for understanding the mechanism of this complex transcriptional machine. We show that the genome segments maintain local order during transcription.

PMID:
10338212
DOI:
10.1016/s0092-8674(00)80758-8
[Indexed for MEDLINE]
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