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FEBS Lett. 1999 Apr 23;449(2-3):277-83.

Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein.

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Genome Information Research Center, Osaka University, Suita, Japan.

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  • FEBS Lett. 2015 Oct 7;589(20 Pt B):3220.


We produced transgenic mouse lines that accumulate mutated green fluorescent protein (EGFP) in sperm acrosome, a membrane limited organelle overlying the nucleus. The sperm showed normal fertilizing ability and the integrity of their acrosome was easily examined in a non-invasive manner by tracing the GFP in individual 'live' sperm with fluorescent microscopy. The time required for the dispersal of acrosomal contents was demonstrated to be approximately 3 s after the onset of acrosome reaction.

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