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Biotechniques. 1999 May;26(5):892-4, 896, 898 passim.

Increasing DNA transfer efficiency by temporary inactivation of host restriction.

Author information

1
Department of Microbiology, University of Illinois, Urbana-Champaign 61801, USA.

Abstract

E. coli and Salmonella typhimurium are widely used bacterial hosts for genetic manipulation of DNA from prokaryotes and eukaryotes. Introduction of foreign DNA by electroporation or transduction into E. coli and Salmonella is limited by host restriction of incoming DNA by the recipient cells. Here, we describe a simple method that temporarily inactivates host restriction, allowing high-frequency DNA transfer. This technique might be readily applied to a wide range of bacteria to increase DNA transfer between strains and species.

PMID:
10337482
DOI:
10.2144/99265st02
[Indexed for MEDLINE]
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