Format

Send to

Choose Destination
Nat Struct Biol. 1999 May;6(5):417-21.

Solution structure of the extended neuronal nitric oxide synthase PDZ domain complexed with an associated peptide.

Author information

1
Department of Biochemistry, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, PR China.

Abstract

The PDZ domain of neuronal nitric oxide synthase (nNOS) functions as a scaffold for organizing the signal transduction complex of the enzyme. The NMR structure of a complex composed of the nNOS PDZ domain and an associated peptide suggests that a two-stranded beta-sheet C-terminal to the canonical PDZ domain may mediate its interaction with the PDZ domains of postsynaptic density-95 and alpha-syntrophin. The structure also provides the molecular basis of recognition of Asp-X-Val-COOH peptides by the nNOS PDZ domain. The role of the C-terminal extension in Asp-X-Val-COOH peptide binding is investigated. Additionally, NMR studies further show that the Asp-X-Val-COOH peptide and a C-terminal peptide from a novel cytosolic protein named CAPON bind to the same pocket of the nNOS PDZ domain.

Comment in

PMID:
10331866
DOI:
10.1038/8216
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center