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Immunology. 1999 Apr;96(4):524-8.

Direct evidence that ERK regulates the production/secretion of interleukin-2 in PHA/PMA-stimulated T lymphocytes.

Author information

1
Department of Immunopathology, Womens' and Children's Hospital, North Adelaide, Australia.

Abstract

Although p21ras, raf-1 and MEK have been shown to regulate directly the transcriptional activity of NFAT (nuclear factor of activated T cells) and/or the interleukin-2 (IL-2) promoter, direct evidence that the extracellular signal-regulated protein kinase (ERK) is involved in regulating IL-2 production is still lacking. Here, we demonstrate that transfection of Jurkat cells with a dominant negative mutant of ERK1 (Erk1-K71R) resulted in the suppression of mitogen-stimulated production/secretion of IL-2. This was accompanied by a parallel inhibition of mitogen-stimulated ERK activity. These data provide direct evidence, for the first time, that ERK plays a vital role in regulating the production/secretion of IL-2.

PMID:
10233737
PMCID:
PMC2326783
DOI:
10.1046/j.1365-2567.1999.00724.x
[Indexed for MEDLINE]
Free PMC Article

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