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Carbohydr Res. 1998 Dec 21;314(1-2):101-14.

alpha-D-Glcp-(1<-->1)-beta-D-Galp-containing oligosaccharides, novel products from lactose by the action of beta-galactosidase.

Author information

1
Department of Bio-Organic Chemistry, Utrecht University, Netherlands.

Abstract

A mixture of oligosaccharides produced by beta-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [beta-D-Galp-(1-->4)]n-alpha-D-Glcp- (1<-->1)-beta-D-Galp[-(4<--1)-beta-D-Galp]m, with n, m = (1, 2, 3, or 4) and beta-D-Galp-(1-->2)-alpha-D-Glcp- (1<-->1)-beta-D-Galp.

PMID:
10230039
DOI:
10.1016/s0008-6215(98)00285-7
[Indexed for MEDLINE]

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