Abstract
The mouse NKR-P1 family includes at least three genes: NKR-P1A, -B, -C. Neither surface expression nor function of the NKR-P1B gene product has previously been shown. Here, we demonstrate that the SJL/J allele of the NKR-P1B gene product is expressed on SJL/J NK cells, and is recognized by PK136 mAb. Interestingly, the same mAb does not recognize the NKR-P1B gene product of C57BL/6. We have also generated a novel mAb, 1C10, that recognizes an activation receptor on SJL/J NK cells. Activation of the NKR-P1B receptor-inhibited 1C10 mAb induced redirected lysis and recruited SHP-1, indicating that NKR-P1B is an inhibitory receptor. Therefore, the mouse NKR-P1 gene family, like the Ly49 family, includes both activation and inhibitory receptors.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal
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Antibody Specificity
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Antigens
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Antigens, Surface / isolation & purification*
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Clone Cells
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Cytotoxicity, Immunologic
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Hybridomas
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Intracellular Signaling Peptides and Proteins
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Killer Cells, Natural / immunology*
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Lectins, C-Type*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C3H
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Mice, Inbred C57BL
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Mice, Inbred DBA
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Molecular Sequence Data
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NK Cell Lectin-Like Receptor Subfamily B
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Protein Tyrosine Phosphatase, Non-Receptor Type 11
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Protein Tyrosine Phosphatases
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Proteins
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Receptors, Immunologic / isolation & purification*
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Sequence Analysis
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Sequence Homology, Amino Acid
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Species Specificity
Substances
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Antibodies, Monoclonal
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Antigens
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Antigens, Surface
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Intracellular Signaling Peptides and Proteins
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Lectins, C-Type
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NK Cell Lectin-Like Receptor Subfamily B
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Proteins
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Receptors, Immunologic
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Protein Tyrosine Phosphatase, Non-Receptor Type 11
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Protein Tyrosine Phosphatases
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Ptpn11 protein, mouse
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Ptpn6 protein, mouse