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FEMS Microbiol Lett. 1999 Apr 15;173(2):403-9.

Identification of Tn10 insertions in the dsbA gene affecting Escherichia coli biofilm formation.

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1
Department of Molecular Microbiology, Faculty of Biology, Free University of Amsterdam, The Netherlands. pierre@bio.vu.nl

Abstract

Escherichia coli was used as model to study initial adhesion and early biofilm development to an abiotic surface. Tn10 insertion mutants with reduced attachment to a polystyrene surface were isolated. Three adhesion mutants harbored the transposon in the dsbA gene, whose product, DsbA, catalyses folding of numerous extracytoplasmic disulfide bond-containing proteins. All three mutants were weakly adherent and grew poorly. Cell surface structure analysis showed that motility. type 1 fimbriation and lipopolysaccharide structure were affected in these mutants. The pleiotropic effect of the dsbA mutations on biofilm formation is discussed.

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