Comparative physical and genetic maps of the virulence plasmids of Salmonella enterica serovars typhimurium, enteritidis, choleraesuis, and dublin

Infect Immun. 1999 May;67(5):2611-4. doi: 10.1128/IAI.67.5.2611-2614.1999.

Abstract

Using fragment profiling, PCR, and Southern hybridization, we found that Salmonella enterica serovar Choleraesuis harbored virulence plasmids of various sizes, whereas serovars Typhimurium, Enteritidis, and Dublin carried a plasmid of a unique size. Also, the virulence plasmid of Typhimurium contained genes in the same order detected in the other three plasmids, all of which contained deletions.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • DNA Primers / genetics
  • Genes, Bacterial
  • Physical Chromosome Mapping
  • Plasmids / genetics*
  • Polymerase Chain Reaction
  • Salmonella enterica / classification
  • Salmonella enterica / genetics*
  • Salmonella enterica / pathogenicity*
  • Salmonella enteritidis / genetics
  • Salmonella enteritidis / pathogenicity
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / pathogenicity
  • Serotyping
  • Virulence / genetics

Substances

  • DNA Primers