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Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4832-7.

Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells.

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1
Section of Microbiology, Cornell University, Ithaca, NY 14853, USA.

Abstract

TraR is an Agrobacterium transcriptional regulator whose activity requires the pheromone N-3-oxooctanoyl-L-homoserine lactone. TraR was purified as a complex with the pheromone and contained one pheromone molecule per protein monomer. TraR-pheromone complexes bound to a single DNA site and activated two promoters that flank this site. Promoter expression was elevated 30-fold by using a supercoiled template. Pheromone binding increased the affinity of TraR for this binding site. Pheromone also increased TraR abundance in vivo by causing a 20-fold decrease in TraR turnover rates.

PMID:
10220379
PMCID:
PMC21777
[Indexed for MEDLINE]
Free PMC Article
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