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J Interferon Cytokine Res. 1999 Mar;19(3):235-41.

Production of interleukin-12 as a self-processing 2A polypeptide.

Author information

1
Institute for Animal Health, Compton, Nr. Newbury, Berkshire, UK. paul.chaplin@aahl.dah.csiro.au

Abstract

Interleukin-12 (IL-12) is a heterodimeric cytokine composed of two disulfide-linked subunits (p40 and p35) encoded by separate genes. We used the apparent autocleavage property of a 2A peptide from the foot-and-mouth disease virus (FMDV) to express bovine (Bo) IL-12 as a self-processing polypeptide (p402Ap35). We demonstrate that 2A will mediate the cleavage of p402Ap35 into two separate subunits in a manner similar to that observed during the processing of the FMDV polypeptide. Furthermore, this 2A polypeptide encoded a functional heterodimer, which elicited activities associated with IL-12 in other species. We propose that this strategy of self-processing polypeptides may be used in many applications where the coordinated and stoichiometric expression of complex proteins is required.

PMID:
10213462
DOI:
10.1089/107999099314162
[Indexed for MEDLINE]

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