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Biochim Biophys Acta. 1999 Apr 14;1445(1):142-7.

Identification and analysis of the promoter region of the human NeuroD-related factor (NDRF)1.

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Third Department of Internal Medicine, Hiroshima University, School of Medicine, 1-2-3 Kasumi, Minami-Ku, Hiroshima 734-8551, Japan.


We isolated and characterized the human NeuroD-related factor (NDRF)/NeuroD2/KW8 gene. The NDRF gene consisted of two exons and one intron. NDRF had one transcriptional starting point, and in its 5'-flanking region, no TATA box was detected, but 16 E boxes (CANNTG) were present. RNA blotting analysis revealed that HIT-T15 and D283 cells expressed a 3.3 kb band of the NDRF transcript. Promoter analysis by luciferase assay demonstrated that luciferase activity changed between -120 and -195, and between -451 and -564, both regions wherein a single E box existed. Radiation hybrid mapping showed that NDRF linked the marker SHGC-36242 with a LOD score of 8.53 and was located on 17q12-22.

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