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J Immunol. 1999 Apr 1;162(7):3757-60.

Cutting edge: receptor-mediated endocytosis of heat shock proteins by professional antigen-presenting cells.

Author information

1
Abteilung Immunologie, Institut für Zellbiologie, Universität Tübingen, Germany.

Abstract

Immunization with heat shock proteins (HSPs) induces Ag-specific CTL responses. The specificity of the immune response is based on peptides associated with HSPs. To investigate how exogenous HSP/peptide complexes gain access to the MHC class I-restricted Ag presentation pathway, we incubated the monocytic cell line P388D1 and the dendritic cell line D2SC/1 with gold-labeled HSPs gp96 and HSC70. We show that HSPs bind specifically to the surface of these APCs and are internalized spontaneously by receptor-mediated endocytosis, demonstrating the existence of specific receptors for HSPs on these cells. In addition, we observe colocalization of internalized HSPs and surface MHC class I molecules in early and late endosomal structures. These findings provide possible explanations for the immunogenicity of HSP/peptide complexes and for the transfer of HSP-associated peptides onto MHC class I molecules.

PMID:
10201889
[Indexed for MEDLINE]
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