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Electrophoresis. 1999 Feb;20(2):418-28.

The measurement of ubiquitin and ubiquitinated proteins.

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Tumor Cell Biology Section, Medicine Branch, National Cancer Institute, National Institutes of Health, Key West Center, Rockville, MD 20850, USA.


Ubiquitination of key cellular proteins involved in signal transduction, gene transcription and cell-cycle regulation usually condemns those proteins to proteasomal or lysosomal degradation. Additionally, cycles of reversible ubiquitination regulate the function of certain proteins in a manner analogous to phosphorylation. In this short review we describe the current methodology for measuring ubiquitin and ubiquitination, provide examples which illustrate how various techniques have been used to study protein ubiquination, alert the readers of pitfalls to avoid, and offer guidelines to investigators newly interested in this novel post-translational protein modification.

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