Possible involvement of p38 MAP kinase in HSP70 expression induced by hypoxia in rat primary astrocytes

T Uehara; M Kaneko; S Tanaka; Y Okuma; Y Nomura

doi:10.1016/s0006-8993(99)01178-6

Possible involvement of p38 MAP kinase in HSP70 expression induced by hypoxia in rat primary astrocytes

Brain Res. 1999 Mar 27;823(1-2):226-30. doi: 10.1016/s0006-8993(99)01178-6.

Authors

T Uehara¹, M Kaneko, S Tanaka, Y Okuma, Y Nomura

Affiliation

¹ Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.

PMID: 10095033
DOI: 10.1016/s0006-8993(99)01178-6

Abstract

The aim of this study was to elucidate the possible mechanism of HSP induction in response to hypoxia in rat primary astrocytes. Treatment with SB203580, a selective p38 MAP kinase (p38 MAPK) inhibitor, attenuated the increase in HSP70 in a concentration-dependent manner. p38 MAPK was activated in response to hypoxic treatment. These results suggest that p38 MAPK positively regulates hypoxia-induced HSP70 expression in astrocytes.

MeSH terms

Animals
Astrocytes / metabolism*
Brain / cytology
Brain / metabolism
Calcium-Calmodulin-Dependent Protein Kinases / antagonists & inhibitors
Calcium-Calmodulin-Dependent Protein Kinases / physiology*
Cells, Cultured
Enzyme Inhibitors / pharmacology
HSP70 Heat-Shock Proteins / antagonists & inhibitors
HSP70 Heat-Shock Proteins / metabolism*
Hypoxia / metabolism*
Imidazoles / pharmacology
Mitogen-Activated Protein Kinases*
Pyridines / pharmacology
Rats
Rats, Wistar
p38 Mitogen-Activated Protein Kinases

Substances

Enzyme Inhibitors
HSP70 Heat-Shock Proteins
Imidazoles
Pyridines
Calcium-Calmodulin-Dependent Protein Kinases
Mitogen-Activated Protein Kinases
p38 Mitogen-Activated Protein Kinases
SB 203580