Abstract
In permissive cells, human cytomegalovirus encodes the protein US28, a functional CC chemokine receptor. US28 polyadenylated mRNA could be detected by RT-PCR as early as 2 h post-infection. US28 mRNA appeared after major IE1 transcripts (UL123), but before transcripts of the early genes pp65 (UL83) and gB (UL55), and the late gene pp150 (UL32). This temporal appearance indicates that US28 is transcribed earlier than previously reported. Furthermore, US28 mRNA could be detected in semi- and non-permissive cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Line
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Cells, Cultured
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Cycloheximide / pharmacology
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Cytomegalovirus / drug effects
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Cytomegalovirus / genetics*
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Cytomegalovirus / growth & development
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Gene Expression Regulation, Viral / drug effects
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Genes, Immediate-Early / genetics
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Humans
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Open Reading Frames / genetics
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RNA, Messenger / metabolism
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Receptors, CCR2
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Receptors, Chemokine / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Time Factors
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Transcription, Genetic / drug effects
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Transcription, Genetic / genetics*
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Tumor Cells, Cultured
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Viral Proteins / genetics
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Virus Replication
Substances
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CCR2 protein, human
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RNA, Messenger
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Receptors, CCR2
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Receptors, Chemokine
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Viral Proteins
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Cycloheximide