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Diagn Microbiol Infect Dis. 1999 Feb;33(2):75-80.

In vitro pharmacodynamic properties of MK-0991 determined by time-kill methods.

Author information

1
College of Pharmacy, University of Iowa Hospitals and Clinics, Iowa City 52242, USA.

Abstract

MK-0991 has demonstrated activity against a variety of fungal pathogens. We evaluated the MIC endpoint for MK-0991 by reading the endpoint using three methods and comparing these results with minimum fungicidal concentrations and electron micrographs. The concentration that resulted in 80% inhibition of fungal growth compared with control, similar to the endpoint for the azole antifungal agents, provided the most consistent results. Additionally, we investigated the time-kill properties of this agent against two isolates each of Candida albicans, Candida glabrata and Candida tropicalis at concentrations ranging from 0.125 x MIC to 16 x MIC. Kill curves were performed using RPMI buffered with morpholine propanesulfonic acid as growth media. Samples were obtained at predetermined time points over 24 h and plated for colony counting. Fungicidal activity was observed with one isolate of C. albicans, two isolates of C. glabrata, and one isolate of C. tropicalis. MK-0991 displayed concentration-dependent activity, which was fungicidal or fungistatic depending on the isolate tested.

PMID:
10091029
DOI:
10.1016/s0732-8893(98)00130-8
[Indexed for MEDLINE]

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