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J Surg Res. 1999 Apr;82(2):131-6.

Evaluation of a hybrid artificial liver using a polyurethane foam packed-Bed culture system in dogs.

Author information

1
Second Department of Surgery, Kyushu University, Fukuoka, 812-8582, Japan.

Abstract

BACKGROUND AND AIMS:

We developed a polyurethane foam packed-bed culture system of hepatocyte spheroids as a hybrid artificial liver (PUF-HAL), which was effective for recovery from liver failure in rat experiments. In this report, the design of a scaled-up PUF-HAL for dogs is described and evaluated using a dog acute liver failure model.

METHODS:

Warm ischemic liver failure was induced with a portocaval shunt in each dog. The dogs were divided into two groups: (1) a control group (N = 4), in which each dog was attached to a PUF-HAL without hepatocytes for 9 h, and (2) a HAL group (N = 5), in which each dog was attached to a PUF-HAL with hepatocytes. Blood pressure, blood ammonia, blood glucose, serum creatinine, and other parameters related to liver function were compared between the two groups.

RESULTS:

In the HAL group, blood ammonia and serum creatinine levels were significantly lower, and blood pressure and blood glucose levels significantly higher, than those in the control group.

CONCLUSIONS:

The scaled-up PUF-HAL developed for large animals is useful as a liver support system in the dog acute liver failure model.

PMID:
10090820
DOI:
10.1006/jsre.1998.5540
[Indexed for MEDLINE]
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