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Trends Cell Biol. 1999 Feb;9(2):61-5.

Photobleaching GFP reveals protein dynamics inside live cells.

Author information

1
Cell Biology and Cell Biophysics Programme, European Molecular Biology Laboratory, Heidelberg, Germany. jwhite@embl-heidelberg.de

Abstract

Cell biologists have used photobleaching to investigate the lateral mobility of fluorophores on the cell surface since the 1970s. Fusions of green fluorescent protein (GFP) to specific proteins extend photobleaching techniques to the investigation of protein dynamics within the cell, leading to renewed interest in photobleaching experiments. This article revisits general photobleaching concepts, reviews what can be learned from them and discusses applications illustrating the potential of photobleaching GFP fusion proteins inside living cells.

PMID:
10087620
[Indexed for MEDLINE]

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