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Trends Cell Biol. 1999 Feb;9(2):52-6.

Dual-colour imaging with GFP variants.

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Cell Biology and Metabolism Branch, Institute of Child Health and Human Development, Bethesda, MD 20892-5430, USA.


Green fluorescent protein (GFP) has become an important tool in cell biology and is widely used as a reporter for imaging intracellular proteins and structures in live cells. Recently, spectral variants of GFP with red- and blue-shifted fluorescence emissions have been characterized, opening the possibility of double labelling with two different-coloured GFP fusion proteins. This article reviews recent advances in this technique, with special emphasis on time-lapse imaging applications in living cells.

[Indexed for MEDLINE]

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