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Genet Anal. 1999 Mar;15(1):1-4.

A polymerase chain reaction-based screening method for transgenic Arabidopsis.

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1
Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, Belgium.

Abstract

A simple and rapid PCR-based method for screening transformed Arabidopsis plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified.

PMID:
10084120
[Indexed for MEDLINE]
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