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Protein Sci. 1998 Aug;7(8):1839-42.

Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis.

Author information

1
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.

Abstract

A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multi-milligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.

PMID:
10082383
PMCID:
PMC2144084
DOI:
10.1002/pro.5560070821
[Indexed for MEDLINE]
Free PMC Article

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