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J Chromatogr B Biomed Sci Appl. 1999 Feb 19;723(1-2):301-5.

Determination of plasma phenobarbital concentration by high-performance liquid chromatography in rat offspring.

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1
Department of Hospital Pharmacy, Okayama University Medical School, Japan.

Abstract

Plasma phenobarbital (PB) concentrations in rat offspring were determined using a 9 microl capillary by high-performance liquid chromatography (HPLC). Capillary plasma which was put into a Bond Elut cartridge column by using 1 ml of 0.01 M KH2PO4 was applied to the column with 50 microl of 2 microg/ml of acetanilide (internal standard, I.S.). After washing the column, PB and I.S. were eluted with methanol and injected into the HPLC system. There were excellent linear correlation between the amount of PB and length of the capillary at three different concentrations. Calibration for PB was linear in the range of 0-50 microg/ml. The coefficients of variation were 3.4-5.0% and 5.9-7.5% in the within-day and between-day assays, respectively. The extraction recovery rates were 87.5-105.4%. By this method, it was possible to measure plasma PB concentrations in rat offspring without killing. These results suggested that this method is very useful to determine the plasma PB concentration derived from mother's milk in newborn rats.

PMID:
10080659
DOI:
10.1016/s0378-4347(98)00534-9
[Indexed for MEDLINE]

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