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J Clin Invest. 1999 Mar;103(5):597-604.

Modulation of apoptosis by the cyclin-dependent kinase inhibitor p27(Kip1).

Author information

1
Department of Medicine, Division of Nephrology, University of Washington School of Medicine, Seattle, Washington 98195-6521, USA.

Abstract

Proliferation and apoptosis are increased in many types of inflammatory diseases. A role for the cyclin kinase inhibitor p27(Kip1) (p27) in limiting proliferation has been shown. In this study, we show that p27(-/-) mesangial cells and fibroblasts have strikingly elevated rates of apoptosis, not proliferation, when deprived of growth factors. Apoptosis was rescued by restoration of p27 expression. Cyclin A-cyclin-dependent kinase 2 (CDK2) activity, but not cyclin E-CDK2 activity, was increased in serum-starved p27(-/-) cells, and decreasing CDK2 activity, either pharmacologically (Roscovitine) or by a dominant-negative mutant, inhibited apoptosis. Our results show that a new biological function for the CDK inhibitor p27 is protection of cells from apoptosis by constraining CDK2 activity. These results suggest that CDK inhibitors are necessary for coordinating the cell cycle and cell-death programs so that cell viability is maintained during exit from the cell cycle.

PMID:
10074476
PMCID:
PMC408127
DOI:
10.1172/JCI5461
[Indexed for MEDLINE]
Free PMC Article

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