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Annu Rev Med. 1999;50:277-90.

Applications of NMR spectroscopy to study muscle glycogen metabolism in man.

Author information

1
Department of Internal Medicine III, University of Vienna Medical School, Austria. michael.roden@akh-wien.ac.at

Abstract

Prior to the advent of nuclear magnetic resonance (NMR) spectroscopy, human glucose metabolism was studied through tracer and tissue biopsy methodology. NMR spectroscopy now provides a noninvasive means to monitor metabolic flux and intracellular metabolite concentrations continuously. 13C NMR spectroscopy has shown that muscle glycogen synthesis accounts for the majority of insulin-stimulated muscle glucose uptake in normal volunteers and that defects in this process are chiefly responsible for insulin resistance in type 1 and type 2 diabetes mellitus, as well as in other insulin resistant states (obesity, insulin-resistant offspring of type 2 diabetic parents, elevation of plasma FFA concentrations). Furthermore, using 31P NMR spectroscopy to measure intracellular glucose-6-phosphate, it has been shown that defects in insulin-stimulated glucose transport/phosphorylation activity are primarily responsible for the insulin resistance in these states.

PMID:
10073278
DOI:
10.1146/annurev.med.50.1.277
[Indexed for MEDLINE]

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