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Int Immunol. 1999 Feb;11(2):133-41.

Induction of CD8+ T cell-mediated protective immunity against Trypanosoma cruzi.

Author information

1
Department of Parasitology, Juntendo University School of Medicine, Tokyo, Japan.

Abstract

Trypanosoma cruzi was transformed with the Plasmodium yoelii gene encoding the circum-sporozoite (CS) protein, which contains the well-characterized CD8+ T cell epitope, SYVPSAEQI. In vivo and in vitro assays indicated that cells infected with the transformed T. cruzi could process and present this malaria parasite-derived class I MHC-restricted epitope. Immunization of mice with recombinant influenza and vaccinia viruses expressing the SYVPSAEQI epitope induced a large number of specific CD8+ T cells that strongly suppressed parasitemia and conferred complete protection against the acute T. cruzi lethal infection. CD8+ T cells mediated this immunity as indicated by the unrelenting parasitemia and high mortality observed in immunized mice treated with anti-CD8 antibody. This study demonstrated, for the first time, that vaccination of mice with vectors designed to induce CD8+ T cells is effective against T. cruzi infection.

PMID:
10069411
DOI:
10.1093/intimm/11.2.133
[Indexed for MEDLINE]

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