Format

Send to

Choose Destination
J Chromatogr B Biomed Sci Appl. 1999 Feb 5;722(1-2):225-54.

Single cell gel electrophoresis assay: methodology and applications.

Author information

1
Departamento de Genética y Toxicología Ambiental, Instituto de Investigaciones Biomedicas, UNAM, Mexico DF, Mexico.

Abstract

The single cell gel electrophoresis or Comet assay is a sensitive, reliable, and rapid method for DNA double- and single-strand breaks, alkali-labile sites and delayed repair site detection, in eukaryotic individual cells. Given its overall characteristics, this method has been widely used over the past few years in several different areas. In this paper we review the studies published to date about the principles, the basic methodology with currently used variations. We also explore the applications of this assay in: genotoxicology, clinical area, DNA repair studies, environmental biomonitoring and human monitoring.

PMID:
10068143
DOI:
10.1016/s0378-4347(98)00313-2
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center