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Curr Opin Microbiol. 1998 Aug;1(4):406-10.

Green fluorescent protein as a reporter of transcription and protein localization in fungi.

Author information

1
Department of Molecular Biology, Johns Hopkins Medical School, Preclinical Teaching Building 522, 725 North Wolfe Street, Baltimore MD 21205-2185, USA. bcormack@bs.jhmi.edu

Abstract

Green fluorescent protein (GFP) is a versatile and powerful tool for analysis of diverse biological processes. The recent development of GFP variants with altered spectral properties and altered codon composition has allowed efficient expression of GFP in a number of fungal species. GFP has been successfully used to analyze transcription regulation as well as protein and organelle localization, and promises to give an unprecedented view into the dynamic subcellular processes that shape the fungal cell.

PMID:
10066516
[Indexed for MEDLINE]

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