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Protein Eng. 1999 Jan;12(1):41-5.

Analysis of protein-protein interactions by mutagenesis: direct versus indirect effects.

Author information

1
MRC Unit for Protein Function & Design, Cambridge IRC for Protein Engineering, University Chemical Laboratory, UK.

Abstract

Site-directed mutagenesis, including double-mutant cycles, is used routinely for studying protein-protein interactions. We now present a case analysis of chymotrypsin inhibitor 2 (CI2) and subtilisin BPN' using (i) a residue in CI2 that is known to interact directly with subtilisin (Tyr42) and (ii) two CI2 residues that do not have direct contacts with subtilisin (Arg46 and Arg48). We find that there are similar changes in binding energy on mutation of these two sets of residues. It can thus be difficult to interpret mutagenesis data in the absence of structural information.

PMID:
10065709
[Indexed for MEDLINE]

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